Table 2.
Residue | Sample | Rhamnose | Fucose | Arabinose | Xylose | Mannose | Galactose | Glucose | Mass |
Ammonium oxalate | WT | 0.1 ± 0.0 | ND | 0.3 ± 0.0 | 0.2 ± 0.0 | 0.1 ± 0.0 | 0.3 ± 0.0 | 2.0 ± 0.1* | 35.0 ± 2.0 |
bc14 | 0.1 ± 0.0 | ND | 0.3 ± 0.0 | 0.4 ± 0.0 | 0.1 ± 0.0 | 0.3 ± 0.0 | 0.7 ± 0.0* | 37.4 ± 1.4 | |
pBC14F | 0.1 ± 0.0 | ND | 0.3 ± 0.0 | 0.2 ± 0.0 | 0.1 ± 0.0 | 0.3 ± 0.0 | 3.0 ± 0.2* | 34.2 ± 3.1 | |
1 N KOH soluble | WT | 0.4 ± 0.0 | 0.2 ± 0.0 | 16.2 ± 0.6* | 120.7 ± 3.9* | 0.2 ± 0.0 | 4.6 ± 0.2 | 17.0 ± 0.6* | 289.8 ± 53.6 |
bc14 | 0.5 ± 0.0 | 0.2 ± 0.0 | 30.6 ± 1.0* | 171.3 ± 5.5* | 0.5 ± 0.0 | 7.5 ± 0.3 | 6.9 ± 0.3* | 361.3 ± 7.6 | |
pBC14F | 0.4 ± 0.0 | 0.2 ± 0.0 | 17.6 ± 0.3* | 124.4 ± 1.9* | 0.3 ± 0.0 | 5.0 ± 0.1 | 36.7 ± 0.6* | 273.2 ± 57.2 | |
4 N KOH soluble | WT | 0.2 ± 0.0 | 0.1 ± 0.0 | 4.5 ± 0.5* | 34.5 ± 3.5* | 0.1 ± 0.0 | 2.2 ± 0.2 | 9.3 ± 1.1* | 64.0 ± 4.4 |
bc14 | 0.2 ± 0.0 | ND | 3.8 ± 0.3* | 20.0 ± 1.6* | 0.1 ± 0.0 | 1.7 ± 0.1 | 5.0 ± 0.3* | 50.4 ± 3.1 | |
pBC14F | 0.2 ± 0.0 | 0.1 ± 0.0 | 3.5 ± 0.3* | 23.0 ± 1.9 | 0.1 ± 0.0 | 1.9 ± 0.2 | 11.4 ± 1.0* | 58.0 ± 4.0 | |
4 N KOH insoluble | WT | ND | ND | 0.4 ± 0.0 | 1.9 ± 0.1 | ND | 0.2 ± 0.0 | 9.9 ± 0.5* | ND |
bc14 | 0.1 ± 0.0 | ND | 0.7 ± 0.0 | 2.1 ± 0.1 | 0.1 ± 0.0 | 0.4 ± 0.0 | 7.7 ± 0.2* | ND | |
pBC14F | ND | ND | 0.4 ± 0.0 | 1.8 ± 0.1 | 0.0 ± 0.0 | 0.3 ± 0.0 | 10.2 ± 0.3* | ND |
ND, not detected. The amounts of monosaccharides are determined by GC-MS analysis of alditol acetate derivatives.
*Significantly different (t test at P < 0.05) compared with WT (n = 4) ± SD.