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. 2011 Mar 7;108(12):4980–4985. doi: 10.1073/pnas.1102198108

Fig. 3.

Fig. 3.

Mutant merlin protein maintains intrinsic functional capacity. (A) Immunofluorescence staining for F-actin (green) and merlin mutants (red) in CH157 NM cells transfected with wild-type merlin, merlin mutants, as well as empty vector (blank). The nuclei of cells were counterstained with Hoechst (blue). Arrows point to F-actin stress fibers and arrowheads point to mutant merlin. (B) Western blot of lysates from CH157 NM cells transfected with either wild-type or K413E mutant merlin protein. K413E-transfected cells demonstrate increased merlin ubiquitylation and up-regulation of β-catenin and cyclin D1 cytoplasmic expression. (C) MTT proliferation assay demonstrating suppression of CH157 NM cell growth with mutant merlin transfection compared with empty vector (blank). (D) Flow cytometric BrdU-PI cell cycle analysis demonstrating reduced proliferation in CH157 NM cells transfected with L46R and A211D merlin mutants compared with wild type and empty vector (blank).