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. 2011 Mar 7;108(12):5015–5020. doi: 10.1073/pnas.1016933108

Fig. 5.

Fig. 5.

Heart defects in B-Raf+/LSLV600E mice. (A) Histological analysis of heart chambers and aortic valves of 8-wk-old B-Raf+/+ and B-Raf+/LSLV600E littermates. (Left) H&E staining of atrial cardiomyocytes. (Scale bar, 100 μm.) (Center) H&E staining of ventricular cardiomyocytes. (Scale bar, 50 μm.) (Right) H&E staining of aortic valves (arrowheads). (Scale bar, 500 μm.) (B) Sirius red staining of ventricular cardiomyocytes of B-Raf+/+ and B-Raf+/LSLV600E littermates. (Scale bar, 100 μm.) (C) Relative cardiomyocyte size. (D) Relative number of cardiomyocytes per area. (E–G) PET analysis of heart functions including (E) end systolic volume, (F) end diastolic volume, and (G) ejection fraction. B-Raf+/+ (open bars) and B-Raf+/LSLV600E (solid bards) mice (n = 6) had a B6 (A–D) or B6/CD1 (E–G) genetic background. For each mouse, three photos of the ventricular area were taken using the same magnification (20×). The number of cardiomyocytes was determined by counting the nuclei. The area occupied by the cardiomyocytes was determined using Image J software. Relative values represented in C and D were obtained by normalizing with those values obtained from control B-Raf+/+ animals. Error bars represent SEMs. *P < 0.05; **P < 0.01.