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ELISPOT detection of swine reactivity to allogeneic PC14 peptide 3. Representative IFN-γ ELISPOT wells by using 3 × 105 pig responder splenocytes per well plus PC14 peptide 3 (50 μg/ml) are shown. Responder splenocytes were harvested from peptide-immunized CyA-treated rejecter pig no. 14071 (Top), unimmunized acute rejecter pig no. 13384 (Middle), and a naïve pig (Bottom).
