Table 1.

Primer sequences used in reverse transcriptase polymerase chain reaction (RT-PCR)

Gene	Size (Bp)	Sequence
MMP-2	605	forward 5′-CAGTTTTTCAAGACAGA TCATAAGCG-3′, reverse 5′-TGCTCCTGCTTGAG-ATGTCG-3′
MMP-9	266	forward 5′-AAGCACATGCAGAATGAGTACCG-3′, reverse 5′-GTGGGACAGCTTCTGGTCGAT-3′;
β-Actin	550	forward 5′-ATGAAGATCCTGACCGAGCGT-3′, reverse 5′-AACGCAGCTCAGTAACAGT-CCG-3′.
TGF-α	373	forward 5′ ATgTTgTTCCCTgCAAgTCC 3′, reverse 3 ’ACTATGGAGAGGGGTCGCTT 5′
TGF-β1	661	forward 5′ gAAgTCACCCgCgTgCTAATgg 3′, reverse 3′ GGATGTAAACCTCGGACCTGTGTG 5′

Abbreviations: MMP, matrix metalloproteinase; TGF-α, transforming growth factor-alpha; TGF-β1, transforming growth factor-beta1; Bp, base pair.