Figure 3.

A) Hematoxylin and eosin-stained representative kidney sections. Control-treated obese ZSF1 rat with severe nephropathy; TRC150094 6 mg/kg body weight and 12 mg/kg body weight-treated rat, respectively, with reduced grade of nephropathy. (Red arrows showing dilated tubules and blue arrows showing hyaline cast). (100× magnification) B) PAS-stained kidney sections. Glomerulus showing moderate glomerulosclerosis in control-treated and TRC150094 6 mg/kg body weight-treated rat, illustrated by global expansion of mesangial matrix (pink stained, yellow arrow), thickened basement membrane (black arrow) with infiltration of foam cells (green arrow). Glomerulus of 12 mg/kg showed milder glomerulosclerosis characterized by expansion of mesangial matrix (pink stained, yellow arrow) and normal capillaries and glomeruli (blue arrow) portion indicating improvement in nephropathy. (×400) For details refer to supplementary data.

Abbreviation: PAS, periodic acid Schiff.