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X-Band EPR spectra of α-syn, mutants and truncated species. Vertical lines correspond to the parallel hyperfine features of wild type α-syn. α-Syn(1–97) gives a spectrum that superimposes on wild type, but all other species show significant variation. α-Syn(1–10) and α-syn(H50A) gives equivalent spectra, but distinct from wild type, demonstrating involvement of His50 in the coordination sphere.
