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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Exp Eye Res. 2010 Dec 4;92(2):147–154. doi: 10.1016/j.exer.2010.11.010

Figure 1. Effect of hGSTA4 transfection on the toxicity of 4-HNE and H2O2 in RPE cells.

Figure 1

RPE cells were transiently transfected with empty p-Target vector (VT) and p-Target vector containing the ORF of hGSTA4 sequence (hGSTA4-Tr). VT and hGSTA4-Tr cells were treated with 0–80 µM of 4-HNE (A) or 0–1000 µM H2O2 (B) for 12 h and assayed for cytotoxicity by MTT assay. The plots show the percent cell survival (mean ± SD, n = 4) at different concentrations of 4-HNE. Over-expression of hGSTA4 in transfected cells was confirmed by western blot analysis (C).