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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Exp Eye Res. 2010 Dec 4;92(2):147–154. doi: 10.1016/j.exer.2010.11.010

Figure 3. Effect of 4-HNE on p-EGFR and EGFR expression in RPE cells.

Figure 3

Cell extracts prepared as described in methods section containing 30 µg protein were subjected to Western blot analysis. (A). Concentration dependent effect of 4-HNE on the expression of EGFR and p-EGFR. (B). Time dependent effect of 5 µM 4-HNE on the expression of EGFR and p-EGFR. (C). Effect of 4-HNE on the expression of EGFR and p-EGFR in VT and hGSTA4-Tr RPE cells. Western blot analysis was performed with total cell lysates using indicated antibodies. GAPDH was used to control for loading differences. The densitometry data in bar graphs below the respective figures represents the mean ± SD (n =3).