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. Author manuscript; available in PMC: 2011 Dec 8.
Published in final edited form as: Mol Biosyst. 2010 Oct 4;6(12):2392–2402. doi: 10.1039/c0mb00115e

FIG. 3.

FIG. 3

Pep1 and Pep2 do not compete with TGF-β in binding to either TβRI-ED or TβRII-ED. (A) Binding of TGF-β1 (41 pM to 30 nM) to TβRII-ED was tested using SPR. TβRI-ED and TβRII-ED were immobilized through their lysine residues. A protein-free flow cell was used as control. TGF-β binds to TβRII-ED with a saturating concentration of 10 nM. At the concentrations tested, TGF-β1 has no observable affinity to TβRI-ED (data not shown). (B) Pep1 does not compete with TGF-β in binding to TβRII-ED. (C) TGF-β1 initiated luciferase gene expression in an Mv1Lu reporter cell line stably transfected with a SBE(CAGA)12-luciferase reporter gene. TβRI kinase inhibitor SB-431542 inhibited TGF-β regulated luciferase gene expression. Pep1 and (D) Pep2 do not alter the cellular response to TGF-β1. In this competition assay, 10 pM TGF-β1 was used.