Table 3. Genome Sequencing and de novo Assembly Statistics.

Strain Name	Median Depth Of Coverage	Number Of Large Contigs	N50 Contig Size	Largest Contig Size	Percent Q40 Plus Bases	Number of Contigs	Number Of Bases	NCBI Accession numberf	NCBI project ID
1942 a	32a	n/a	n/a	n/a	99.94a	n/a	4168266b	CP002207	46075
Detrick-1	30	33	193257	809459	99.93	38	4130179	AEFP00000000	46077
Detrick-2	29	50c	152641	273988c	99.57	67c	4127403	AEFQ00000000	46079
Detrick-3	42	32	193246	809488	99.96	37	4131174	AEFR00000000	46081
Dugway	49	38	205337	419767	99.85	53	4130651	AEFO00000000	34819
ATCC 9372-1	44	29	255796	943057	99.91	36	4108235d	AEFM00000000	37683
ATCC 9372-2	42	34	250623	546945	99.88	40	4130094	AEFU00000000	46211
BACI051-N	47	78	86661	212948	99.95	81	4130502	AEFY00000000	51595
BACI051-E	36	35	192166	630535	99.97	42	4130076	AEFX00000000	48615
1013-1	31	30	282215	914719	99.95	39	4130375	AEFS00000000	46207
1013-2	30	33	225488	512878	99.93	40	4057611e	AEFT00000000	46209
ATCC 49822-1	37	35	192016	638113	99.92	50	4132165	AEFV00000000	46283
ATCC 49822-2	34	34	213362	638120	99.89	51	4135194	AEFW00000000	46285
NRS-356	44	42	178925	527969	99.97	44	4128298	PENDING	PENDING

^aGenome was finished and closed to a single contig. Depth of coverage and Q40+ metrics are for de novo assembly of 454 data only.

^b454Draft sequences appear smaller than 1942 due to collapse of repeat regions.

^cThe average read length of this sample was considerably lower (239 versus >300) for other genomes in this dataset, resulting in larger number of contigs and decreased assembly quality.

^dDeletionof 23 kb with clear join points (positions 4,022,138–4,045,817) verified by optical mapping (Figure 4B).

^eDeletion of 73 kb with clear join points (positions 3,992,613 to 4,065,341) verified by optical mapping (Figure 4B).