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. 2011 Mar 25;6(3):e18087. doi: 10.1371/journal.pone.0018087

Figure 2. HDAC inhibitor reduced the expression of SGLT1 and decreased glucose uptake.

Figure 2

(A) HCT116 cells were treated with 1 µM TSA for 24, 36 or 48 hours. Whole cell lysate were prepared and subjected to western blot analysis with antibodies specific for SGLT1, EGFR and Actin. (B) Cells were cultured in DMEM with 1 mg/ml and treated with 1 µM TSA for 24 hours. The glucose content was measured as described in material and method (Triplicate samples were used in each group. The asterisk indicates a significant difference with p<0.05. Error bars indicate mean ± SE.) (C) Cells were cultured in DMEM with 1 mg/ml or 4.5 mg/ml glucose and treated with 1, 3 or 5 µM TSA for 24 hours. The glucose content was measured. (D) Cells were cultured in DMEM with 1 mg/ml glucose and treated with 1 or 3 µM TSA. After 24 or 48 hours of treatment, the glucose was adjusted to 4.5 mg/ml. The cell survival was measured by MTT assay after 72 hours treatment with TSA. Results were expressed as mean ± SE of three independent experiments performed in triplicate.