Figure 3. Loss of EGFR contributed to HDAC inhibitor-mediated antitumor effects.

(A) HCT116 cells were transfected with Myc-EGFR as well as its vector control and transfected cells were treated with 5 µM SAHA for 24 hours. Cells were fixed by 70% ethanol and stained with propidium iodide, and fraction of cell cycle was analyzed by flow cytometer. (B) HCT116 cells were transfected with Myc-EGFR as well as its vector control and the transfected cells were treated with 5 µM SAHA for 24 hours. Whole cell lysate were prepared and subjected to western blot using Ab specific for EGFR, Myc, p21 and tubulin.