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. 2011 Mar 25;6(3):e18087. doi: 10.1371/journal.pone.0018087

Figure 5. SP1 is essential for the EGFR transcription.

Figure 5

(A) Cells were treated with 1 µM Mithramycin A (MTM) for 18 or 36 hours, then the total cell lysate were prepared. Cells were transfected with 0, 400 or 800 pmole SP1 siRNA, then the total cell lysates were prepared and subjected to western blot analysis with antibodies specific for EGFR and SP1. (B) Cells were transfected with EGFR-Luc and then treated with 0.1, 1 or 5 µM Mithramycin A (MTM) for 16 hours. Luciferase activities were measured as described under “Material and Method”. The results were normalized to the Renilla luciferase activity and expressed as the mean ± SE. For three independent experiments performed in triplicate.