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. 2010 Dec 22;13(2):155–164. doi: 10.1093/neuonc/noq176

Fig. 4.

Fig. 4.

Synergistic induction of cell death by APO010 and TMZ. LNT-229 cells were treated with TMZ for 96 hours followed by APO010 for another 20 hours. The graphs show the results of treatment with either agent alone, the predicted effect assuming independent (additive) effects, and the truly observed effect. The bars express the percentage of living cells as assessed by flow cytometry after annexin V and PI staining. The data are expressed as mean and SEM from 3 independent experiments. We studied LNT-229.neo control cells (TMZ 90 μM) (A), MGMT-transfected cells (TMZ 90 μM) (B), MGMT-transfected cells additionally treated with O6BG (50 µM; TMZ 90 μM) (C), and LN-308 cells (TMZ 600 μM) (D). (E) LNT-229 cells were pretreated with TMZ (90 μM) as indicated above followed by addition of APO010 and assessed at 6 hours for DEVD-amc–cleaving caspase activity. Data are expressed relative to untreated controls as mean and standard deviation (n = 3). One representative out of the 3 independent experiments with similar results is shown.