Table 1.
APO010 induces apoptotic cell death in ex vivo human glioma cell cultures
| SFI | Apoptotic cells [%] ± SEM |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| APO010 |
APO010 + zVAD-fmk (1000 ng/mL) | sCD95L (1000 ng/mL) | CH11 (1000 ng/mL) | ||||||
| 0 ng/mL | 1 ng/mL | 10 ng/mL | 100 ng/mL | 1000 ng/mL | |||||
| TU 113 | 2.8 | 4 ± 0.4 | 10 ± 4.2 | 67 ± 7.8 | 89 ± 1.8 | 92 ± 2.8 | 7 ± 2.1 | 77 ± 4.2 | 11 ± 1.4 |
| TU 159 | 1.4 | 7 ± 0.8 | 7 ± 1.2 | 11 ± 2.4 | 19 ± 3.1 | 21 ± 2.2 | 5 ± 1 | 9 ± 2.4 | 7 ± 0.7 |
| TU 408 | 1.7 | 7 ± 0.7 | 12 ± 2.5 | 18 ± 0.9 | 45 ± 6.1 | 52 ± 5.7 | 9 ± 1.2 | 28 ± 0.3 | 10 ± 0.3 |
| TU 409 | 1.6 | 9 ± 1.7 | 9 ± 1.4 | 30 ± 6.8 | 61 ± 10.2 | 69 ± 7.5 | 8 ± 0.3 | 41 ± 0.3 | 9 ± 0.9 |
| TU 426 | n.d. | 10 ± 2.4 | 19 ± 4.9 | 49 ± 13.6 | 63 ± 9.6 | 69 ± 9.2 | 9 ± 1.9 | 28 ± 4.1 | 15 ± 2.9 |
| TU 431 | 2.5 | 8 ± 3.5 | 6 ± n.d. | 26 ± n.d. | 64 ± 1.8 | 73 ± 2.1 | 7 ± 1.4 | 34 ± 1.4 | 13 ± 4.9 |
| TU 447 | 1.4 | 10 ± 0.4 | 24 ± 3.2 | 63 ± 1.8 | 69 ± 3.2 | 69 ± 4.2 | 13 ± 3.5 | 53 ± 3.9 | 29 ± 6.7 |
| TU 132 | 1 | 3 ± n.d. | 4 ± n.d. | 4 ± n.d. | 3 ± n.d. | 2 ± n.d. | 3 ± n.d. | 3 ± n.d. | 3 ± n.d. |
| TU 446 | n.d. | 2 ± n.d. | n.d. | n.d. | 75 ± n.d. | 79 ± n.d. | 2 ± n.d. | 57 ± n.d. | 9 ± n.d. |
Cell cultures were generated from freshly resected tumor specimens from 9 randomly selected glioblastoma patients. Cells were treated between passages 4 and 9 as indicated for 20 hours. Apoptosis was assessed by annexin V/PI labeling and flow cytometry. Data are expressed as mean percentage of annexin V positive cells ± SEM (n = 1 − 3; n.d., not defined). CD95 expression was assessed by flow cytometry and SFI values are indicated.