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. 2011 Mar 25;6(3):e18119. doi: 10.1371/journal.pone.0018119

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Proteins were eluted from NHS-treated spores and separated by 2D electrophoresis. Proteins were visualized by silver staining and were identified by LTQ-MS/MS as described in Materials and Methods. (A) Control eluates from spores alone without NHS. (B) Proteins eluted from NHS-treated spores by a chaotropic reagent. Protein identifications by LTQ-tandem MS/MS were indicated in gel B.