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. 2001 Mar 13;98(6):3294–3298. doi: 10.1073/pnas.051604498

Figure 1.

Figure 1

Purification of refolded CD1d–β2m monomers loaded with α-GalCer. FPLC and UV-light absorption profile at 280 nm wave length of a refolding mix containing CD1d and β2m in the presence of α-GalCer. The arrow indicates the CD1d–β2m monomeric complexes. (Inset) SDS/PAGE analysis of proteins eluted between 155 and 170 ml, which demonstrates the presence of two proteins of 33 and 12 kDa, corresponding to unglycosylated CD1d and β2m, respectively.