Figure 3.

Staining of mouse NK1.1 cells by human CD1d–α-GalCer tetramers. (a and c) Splenocytes from wild-type C57BL/6 mice and β₂m^−/− mice were stained with human CD1d–α-GalCer tetramers and antibodies specific to CD3 and NK1.1. (b) Cells in histogram plot were gated on the CD3⁺ CD1d–α-GalCer⁺ tetramer⁺ cell population shown in a. Dashed line shows staining with a control antibody, and the continuous line shows staining with anti-NK1.1 (d) As a negative control, splenocytes from wild-type C57BL/6 mice were stained with CD1d–β-GalCer tetramers and anti-CD3 antibody.