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. Author manuscript; available in PMC: 2012 Jun 20.
Published in final edited form as: Behav Brain Res. 2011 Jan 28;220(1):106–111. doi: 10.1016/j.bbr.2011.01.038

Table 1.

Chronic unpredictable stress paradigm

Day Time of 1st stressor 1st stressor Time of 2nd stressor 2nd stressor
1 10:00 AM Swim stress (4 min) 4:00 PM Lights off (2 h)
2 12:30 PM Lights off (3 h) 7:00 PM Lights on overnight
3 10:00 AM Cold isolation (20 min) 4:00 PM Cage rotation (50 min)
4 11:00 AM Cold isolation (1 h) 2:00 PM Restraint stress (40 min)
5 3:00 PM Lights off (2 h) 5:00 PM Cage rotation (30 min)
6 3:30 PM Swim stress (15 min) 7:00 PM Lights on overnight
7 4:00 PM Restraint stress (1 h) 5:00 PM Lights off (1 h)
8 10:00 AM Cage rotation (20 min) 3:30 PM Cold isolation (15 min)
9 12:00 PM Lights off (2 h) 7:00 PM Lights on overnight
10 10:00 AM Restraint stress (1 h) 2:00 PM Swim stress (5 min)
11 Behavioral testing followed by euthanasia and collection of blood and brain samples.