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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Org Lett. 2011 Mar 8;13(7):1758–1761. doi: 10.1021/ol200288w

Figure 2.

Figure 2

Identification and in vitro reconstitution of NRPS325. (A) Organization of ATEG00325 gene cluster from A. terreus. Highlighting in grey is the 12.4 kb hybrid PKS-NRPS; (B) HPLC analysis (320 nm) of ethyl acetate extracts from the in vitro assays. Each assay contains 10 μM NRPS325 in the presence of different reagents partly shown on the right of the traces. The final concentrations of the different components, when added were: 10 μM ApdA-PKS; 10 μM ApdC; 5 mM thiol; 10 mM amino acid; 2 mM NADPH; 1 mM SAM; and 20 mM ATP. All reactions were performed at room temperature for 12 hours in phosphate buffer (pH = 7.4).