Fig. 5.
Adherence of iha+ L strains and their iha− S derivatives to human intestinal epithelial cells. Strains were grown overnight in DMEM (iron < 0.05 µg ml−1) without shaking and adherence assay was performed as described in Experimental procedures. All bacteria and cells were counted in 10 randomly selected fields and bacteria per cell were averaged. Quantitative differences between adherence of the respective L and S strains were determined using unpaired Student's t-test. Morphological pattern of adherence was evaluated according to Scaletsky et al. (1999).
A and D. Adherence of L and S strains to HCT-8 (A) and Caco-2 cells (D) quantified by numbers of bacteria attached per cell. *P < 0.05; **P < 0.001; NS, not significant. Data are expressed as mean ± standard deviations of number of bacteria attached per cell from three independent experiments.
B, C, E and F. Adherence patterns on HCT-8 (B and C) and Caco-2 cells (E and F) exemplified by strain pair 95L (B and E) and 95S (C and F). Bars represent 10 µm.