Table 2.
Copy numbers and genomic integration sites of TelR-OI in L and S strains.
| Status ofc | Genomic junctions of TelR-OIsd | |||||||
|---|---|---|---|---|---|---|---|---|
| Strain no.a | terb | TelR-OI copy no. | serW | serX | UJ/DJ OI 43 | UJ/DJ OI 48 | OI present | OI integrated in |
| 81L | + | 1 | I | O | −/− | +/+ | 48 | serX |
| 95L | + | 2 | O | O | +/+ | +/+ | 43 + 48 | serW, serX |
| 134L | + | 1 | I | O | −/− | +/+ | 48 | serX |
| 135L | + | 2 | O | O | +/+ | +/+ | 43 + 48 | serW, serX |
| 154L | + | 1 | I | O | −/− | +/+ | 48 | serX |
| 81S | − | 0 | I | O | −/− | +/+ | 48Te | serX |
| 95S | − | 0 | O | O | −/+ | −/+ | 43T + 48T | serW, serX |
| 134S | − | 0 | I | O | −/− | +/+ | 48T | serX |
| 135S | − | 0 | O | O | +/+ | +/+ | 43T + 48T | serW, serX |
| 154S | − | 0 | I | O | −/− | +/+ | 48T | serX |
| EDL933f | + | 2 | O | O | +/+ | +/+ | 43 + 48 | serW, serX |
| Sakaig | + | 1 | I | O | −/− | +/+ | 48 | serX |
| 493/89h | − | 0 | I | I | −/− | −/− | None | n.a. |
L, large, and S, small colony variant of each respective strain.
+, all seven genes of the ter cluster (terZABCDEF) were present; −, all seven genes were absent.
I, the gene is intact; O, the gene is occupied.
+, amplicon size was identical to that from strain EDL933; −, no amplicon was obtained.
T, truncated OI 43 and/or OI 48.
Control strains; data of PCR analyses are in agreement with complete genomic sequences of EHEC O157:H7 strains EDL933 (GenBank Accession No. AE005174), and Sakai RIMD 0509952 (GenBank Accession No. NC_002695); strain 493/89 (sorbitol-fermenting EHEC O157:NM) lacks the ter gene cluster (Bielaszewska et al., 2005) and has serW and serX intact as determined by sequence analysis in this study.
TelR-OI, tellurite resistance-encoding O islands; UJ, upstream (5′) junction; DJ, downstream (3′) junction; n.a., not applicable.