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. 2010 Nov 20;39(6):2432–2444. doi: 10.1093/nar/gkq1051

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Discontinuous hammerheads (HHd) against PSTVd (–) RNA. (A) Diagrams representing the product fraction (F) as a function of time at 10 and 0.1 mM Mg⁺² (left and right, respectively) generated by five different hammerheads: HHd-ELVd-UA/GUGU, HHd-ELVd-UA/UACG, HHd-ELVd-CG/AAAA, HHd-ELVd-CG/CCCC and HHd-sTRSV. F-values represent the mean of two independent experiments. (B) Analysis by denaturing PAGE (5%) and autoradiography of reactions catalyzed by three of the hammerheads at 0.1 mM Mg⁺². The positions and size of the substrate (S) and of the resulting 3′ and 5′ cleavage products (P) are indicated.