Table 1.

Trans-cleavage activity of discontinuous (HHd) and extended (HHe) hammerheads against short RNA substrates

Hammerhead	[MgCl2]	kcat (min−1)a	F∞b
HHd-ELVd-UA/GUGU	10	0.52 ± 0.065	0.92
	1	0.49 ± 0.062	0.90
	0.1	0.03 ± 0.002	0.47
HHd-ELVd-UA/UACG	10	0.36 ± 0.002	0.89
	1	0.31 ± 0.015	0.87
	0.1	0.05 ± 0.003	0.04
HHd-ELVd-CG/AAAA	10	0.52 ± 0.058	0.88
	1	0.42 ± 0.024	0.85
	0.1	0.02 ± 0.003	0.43
HHd-ELVd-CG/CCCC	10	0.18 ± 0.008	0.88
	1	0.13 ± 0.008	0.88
	0.1	n.m.c	0.09
HHd-ELVd-CG/GUGU	10	0.45 ± 0.069	0.88
	1	0.25 ± 0.059	0.89
	0.1	n.m.	0.05
HHd-sTRSV	10	1.41 ± 0.194	0.85
	1	0.53 ± 0.090	0.79
	0.1	0.03 ± 0.003	0.62
HHe-PLMVd	10	1.11 ± 0.148	0.87
	1	0.28 ± 0.051	0.79
	0.1	0.08 ± 0.008	0.79
HHe-ELVd	10	0.41 ± 0.043	0.85
	1	0.07 ± 0.003	0.86
	0.1	0.02 ± 0.003	0.18
HHe-ELVdΔL1	10	0.14 ± 0.010	0.88
	1	0.04 ± 0.003	0.61
	0.1	n.m.	0.00
HHe-sTRSVΔL1	10	0.63 ± 0.028	0.97
	1	0.45 ± 0.031	0.98
	0.1	0.02 ± 0.002	0.48

^aCleavage rate constant.

^bFraction of product at the end point of the reaction.

^cNon-measurable.