Table 2.

Trans-cleavage activity of discontinuous (HHd) and extended (HHe) hammerheads against the full-length PSTVd (–) RNA

Hammerhead	[MgCl2]	ka (min−1)a	kb (min−1)b	F∞c	Fad
HHd-ELVd-UA/GUGU	10	0.08 ± 0.006		0.32
	1	0.03 ± 0.005		0.21
	0.1	n.m.e		0.08
HHd-ELVd-CG/GUGU	10	0.21 ± 0.032	0.005 ± 0.001	0.62	0.45
	1	0.25 ± 0.038	0.009 ± <0.001	0.58	0.34
	0.1	0.06 ± 0.004		0.14
HHd-ELVd-UA/UACG	10	0.76 ± 0.206	0.002 ± 0.001	0.64	0.55
	1	0.18 ± 0.007	0.013 ± 0.002	0.58	0.23
	0.1	0.09 ± 0.002	0.005 ± 0.002	0.24	0.17
HHd-ELVd-CG/AAAA	10	0.33 ± 0.048	0.006 ± 0.001	0.74	0.52
	1	0.26 ± 0.026	0.007 ± <0.001	0.76	0.48
	0.1	0.16 ± 0.074	0.010 ± 0.003	0.29	0.15
HHd-ELVd-CG/CCCC	10	0.18 ± 0.038		0.52
	1	0.11 ± 0.024	0.005 ± 0.001	0.50	0.35
	0.1	0.06 ± 0.005		0.13
HHd-sTRSV	10	0.08 ± 0.013		0.54
	1	0.06 ± 0.007		0.43
	0.1	n.m.		0.09
HHe-PLMVd	10	0.83 ± 0.188	< 0.001 ± 0.001	0.67	0.64
	1	0.77 ± 0.274	< 0.001 ± <0.001	0.6	0.58
	0.1	0.09 ± 0.012	0.004 ± 0.001	0.73	0.57
HHe-ELVd	10	0.27 ± 0.045	0.007 ± <0.001	0.64	0.45
	1	0.08 ± 0.007		0.56
	0.1	0.05 ± 0.004		0.17
HHe-ELVdΔL1	10	0.17 ± 0.011	0.010 ± <0.001	0.63	0.35
	1	0.02 ± 0.003	0.015 ± 0.002	0.61	0.35
	0.1	n.m.		0.00
HHe-sTRSVΔL1	10	0.61 ± 0.133	0.005 ± 0.001	0.76	0.55
	1	0.13 ± 0.009	0.004 ± 0.002	0.73	0.53
	0.1	n.m.	n.m.	0.00

^aCleavage rate constant (in biphasic cleavage reactions refers to the rapid ‘a’ process).

^bCleavage rate constant for the slow ‘b’ process in biphasic cleavage reactions

^cFraction of product at the end point of the reaction

^dFraction of product at the end point of the rapid ‘a’ process in biphasic cleavage reactions.

^eNon-measurable.