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. 2010 Nov 20;39(6):2234–2248. doi: 10.1093/nar/gkq1117

Figure 3.

Figure 3.

Overexpression of PAK1IP1 inhibits BrdU incorporation and induces G1/S cell-cycle delay. (A) Overexpression of PAK1IP1 inhibits BrdU incorporation. HeLa cells were transfected with control, PAK1IP1, P1-mNoLS or P1-dNLS, respectively. The cells were immunostained with BrdU specific antibody (A-1). The percentages of BrdU positive cells in GFP labeled population (A-2) were determined as described in ‘Materials and Methods’ section. The arrows showed the GFP-positive cells with much less BrdU incorporation, and the data were from three independent experiments. (B) Overexpression of PAK1IP1 induces G1/S cell-cycle delay. (B-1), U2OS cells were transfected with or without p53 siRNA respectively, after 12 h, the cells were infected with lentivirus of PAK1IP1 and control vector, then the cells were analyzed by FACS analysis. (B-2), overexpression of PAK1IP1 regulates G1/S phase transition in synchronized cells. The post-infected cells were synchronized with double-thymidine block, and then the cells were collected at the indicated time points after release. Cell-cycle distributions of the cells were analyzed by FACS analysis. The error bars indicate standard deviations from three independent experiments, and the differences in both variables were analyzed for significance by Student’s t-test. P1, PAK1IP1. Thy, thymidine. **P < 0.01 and *P < 0.05. Bars, 20 μm.