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. 2010 Nov 20;39(6):2249–2259. doi: 10.1093/nar/gkq1140

Figure 5.

Figure 5.

NHP6A exchange data. (a) Complex formed from 200 nM NHP6Agfp. (b) Addition of 200 nM wtNHP6A resulted in complete exchange with the bound NHP6Agfp. (c) Re-addition of 200 nM NHP6Agfp resulted in exchange with bound wtNHP6A. (d) NHP6A exchange curves. Each curve is the average of several data sets. Because most of the exchange occurs so quickly (i.e. before the first image could be acquired), the data is presented in terms of absolute fluorescence showing a clear acceleration of off rate after addition of 400 nM wtNHP6A (bottom curve) compared to 0 nM wtNHP6A (top curve). The error in the initial fluorescence point is the standard error of the mean for several data sets.