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. 2011 Jan 10;39(6):e39. doi: 10.1093/nar/gkq1293

Table 1.

PEP assay for compounds dU.I–dU.III

Polymerase	IC₅₀ values			Incorporation bias
Polymerase	dU.I	dU.II	dU. III	dU.I/HOMedUTP	dU.II/HOMedUTP	dU.III/HOMedUTP
Bst	98 ± 13 nM	3.5 ± 0.2 μM	Not Active^a	45	1600	ND
Klenow(exo⁻)	48 ± 4 nM	2.4 ± 0.1 μM	Not Active^a	30	1500	ND
Taq	50 μM^b	Not Active^a	Not Active^a	ND	ND	ND
TaqFS	25 μM^c	Not Active^a	Not Active^a	ND	ND	ND
Therminator	1.8 ± 0.1 nM	1.7 ± 0.2 nM	2.3 ± 0.1 nM	1.1	1.0	1.4
Therminator II	5.7 ± 0.4 nM	14 ± 1 nM	0.14 ± 0.01 μM	0.8	1.9	20
Vent(exo⁻)	2.0 ± 0.1 nM	2.2 ± 0.2 nM	5.9 ± 0.7 nM	1.4	1.6	4.2
DeepVent(exo⁻)	4.9 ± 1.0 nM	17 ± 1 nM	0.22 ± 0.02 μM	1.8	6.3	80

Values in italics represent µM concentrations to contrast those IC₅₀ values in the nM range.

^aNo visible +1 band observed in 377 gel image at 100 μM.

^bConcentration that yielded highest percent incorporation: 45 ± 1%.

^cConcentration that yielded highest percent incorporation: 35 ± 4%;

ND: Not determined.