Figure 5.

i.ln. immunization elicits protective antitumor immunity. Groups of six C57BL/6 mice were immunized three times at 6-day intervals with 10 μg of pEFGPL33A DNA or control pEGFP-N3 DNA. Five days after the last immunization small pieces of solid EL4–33 tumors were transplanted s.c. into both flanks, and tumor growth was measured every 3–4 days. Mean tumor volumes ± 1 SD are shown, and numbers in brackets indicate the ratio of the number of mice with tumors to the total number of mice in a group. One of two similar experiments is shown.