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. 2010 Nov 8;39(6):2103–2115. doi: 10.1093/nar/gkq903

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — The 2D separation of replication intermediates of pUCneo plasmid isolated at 6, 12, 54 and 72 h after Cos-1 cells transfection. Upper panels: replication intermediates digested with EcoRI opposite to the origin. Purified monomers of pUCneo were used for transfection. Middle panel: replication intermediates isolated 6 h after transfection and digested with EcoRI opposite to the origin. Total plasmid sample that consisted of monomers, dimers, and other multimers, was used in this transfection. Lower panel: replication intermediates digested with EcoRI and DpnI. Replication pattern gradually switches from random (Y arc) to origin-based (bubble arc). The Y, bubble and X-shaped recombination products arcs are shown with arrows.