Figure 6.

Unusual replication also takes place in the cells synchronized at G1/S border with mimosine. (A) The 2D electrophoresis of the replication intermediates isolated from Cos-1 cells, which were incubated in the media containing 0.5 mM mimosine and transfected with pUCneo plasmid. Replication intermediates were digested with EcoR1 opposite to the replication origin. In the presence of mimosine only the Y arc corresponding to random replication is visible, while in the absence of mimosine both Y arc and bubble arc is present. (B) Cell cycle analysis by flow cytometry shows that mimosine-treated culture mostly contains cells in G0/G1 phase, and the number of cells in S-phase is significantly less than that in the control.