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. 2010 Nov 8;39(6):2103–2115. doi: 10.1093/nar/gkq903

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Random replication cannot progress through FRA16B region. (A) The scheme of pUCneoFRA16B plasmid. (B) The 2D electrophoresis of the replication intermediates isolated from 293A or Cos-1 cells transfected with pUCneoFRA16B or pUCneoFRARev plasmid. The pUCneoFRARev plasmid contains the FRA16B repeat in the reverse orientation. The intermediates isolated from 293A cells were isolated 6 h after transfection, and digested with EcoRI and HindIII. Replication intermediates isolated from Cos-1 cells 6 h after transfection were digested with EcoRI and HindIII; those isolated 24 h after transfection were digested with EcoRI, HindIII and DpnI.