Skip to main content
. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: J Mol Biol. 2011 Feb 17;407(5):716–730. doi: 10.1016/j.jmb.2011.02.001

Figure 3. Conformation of the actin-binding cleft as a function of temperature in the ADP and APO actomyosin states.

Figure 3

MV FlAsH complexed with IAEDANS-actin in the presence (circles) and absence (squares) of ADP was examined as a function of temperature as in Figure 2. The FRET was monitored by acceptor enhancement and the calculated distance is shown as a function of temperature (relative to the distance at 4 °C). Error bars represent the standard deviation from at least three separate experiments done with three different protein preparations.