The free energies (units are the product of kB, Boltzmann constant, and the temperature, 298° K) of the two actomyosin.ADP states, the collision complex state, and the APO state from the thermodynamic measurements are shown in a reaction coordinate diagram. The conformation of the nucleotide binding pocket (NB pocket) and actin binding cleft (AB cleft) from the FRET measurement is shown for each state. The proposed conformational entropy (conf. S) examined from the thermodynamic analysis and computation simulations is also shown for each state (the AM.ADP* and AM.(ADP) is shown is quotes because this state was not examined by FIRST/FRODA simulations).