Table 1.
Summary of FRET measurements with dmant labeled nucleotides and MV or acto-MV FlAsH.
|
Nucleotide-State |
FRET Efficiency (4°C) |
FRET Efficiency (35°C) |
r (Å) (4°C) |
r (Å) (35°C) |
cFRODA (Å) |
|---|---|---|---|---|---|
| aMV FlAsH:dmantADP | 0.79 ± 0.03 | 0.62 ± 0.06 | 22.8 ± 0.7 | 25.6 ± 1.1 | 27.5 ± 1.0 |
| aActo-MV FlAsH:dmantADP | 0.76 ± 0.02 | 0.66 ± 0.03 | 22.9 ± 0.4 | 25.0 ± 0.5 | NA |
| bMV FlAsH:dmantATP | 0.81 ± 0.05 | 0.74 ± 0.03 | 22.5 ±1.1 | 23.3 ± 0.6 | 23.6 ± 0.6 |
a
FRET efficiency/distance determined from data in Figure 1
b
FRET efficiency/distance determined from stopped-flow fluorescence transients
c
Distance distribution from FIRST/FRODA analysis
a–c
All error bars represent standard deviation of the mean