. Author manuscript; available in PMC: 2012 Apr 15.

Published in final edited form as: J Mol Biol. 2011 Feb 17;407(5):716–730. doi: 10.1016/j.jmb.2011.02.001

Table 2.

Rate and equilibrium constants measured by FRET.

Rate Constant	4°C	10°C	15°C	25°C	30°C	35°C
^ak_+ligand (µM^−1·s⁻¹)	1.5 ± 0.1	2.5 ± 0.1	3.8 ± 0.1	7.6 ± 0.7	9.9 ± 0.6	12.8 ± 0.9
^bk_−ligand (s⁻¹)	1.7± 0.2	3.4 ± 0.4	5.6 ± 1.3	15.4 ± 3.2	22.2 ± 1.7	38.9 ± 2.5
1/K_ligand (µM)	1.1 ± 0.2	1.4 ± 0.2	1.5 ± 0.4	2.0 ± 0.5	2.2 ± 0.3	3.0 ± 0.6
^bk_+pocket (s⁻¹)	0.3 ± 0.1	1.3 ± 0.4	1.2 ± 0.3	1.3 ± 0.4	2.3 ± 1.0	2.0 ± 0.9
^bk_−pocket (s⁻¹)	0.7 ± 0.2	2.4 ± 0.6	3.3 ± 0.7	6.3 ± 1.1	9.7 ± 1.9	12.5 ± 2.6
K_pocket = k_on /k_off	0.4 ± 0.2	0.5 ± 0.2	0.4 ± 0.1	0.2 ± 0.1	0.2 ± 0.1	0.2 ± 0.1
^cK_D overall (µM)	0.8 ± 0.2	0.9 ± 0.2	1.1 ± 0.3	1.7 ± 0.4	1.8 ± 0.3	2.6 ± 0.5
^dK_D overall (µM)	0.8 ± 0.1	1.0 ± 0.1	1.3 ± 0.1	1.3 ± 0.1	1.5 ± 0.1	1.7 ± 0.1

Determined from slope of fast phase in association experiments (Figure 4).

Determined from the average of association and dissociation experiments fit to Eqs 1–3 and 4–6, respectively.

Determined from Eq 7.

Determined by fluorescence titration.