Table 4.
Temperature dependence of MV FlAsH actin-activated ATPase activity.
| Temperature (°C) |
aV0 | bkcat | cKATPase | dk−pocket |
|---|---|---|---|---|
| 4 | 0.04 ± 0.04 | 0.8 ± 0.1 | 2.5 ± 0.5 | 0.8 ± 0.1 |
| 25 | 0.08 ± 0.02 | 6.4 ± 0.3 | 1.4 ± 0.3 | 7.7 ± 0.1 |
| 35 | 0.23 ± 0.22 | 18.8 ± 0.4 | 2.5 ± 0.2 | 19.0 ± 0.1 |
a
ATPase activity in the absence of actin. Errors represent the standard deviation from 3 separate measurements done with three separate protein preparations.
b
Maximum actin-activated ATPase activity determined from fit of data to the Michaelis-Menton relationship. Error bars represent the standard error of the fit of data from three separate protein preparations.
c
Actin concentration at which the ATPase activity is one-half maximal. Error bars represent the standard error of the fit of data from three separate protein preparations.
d
The rate of dmantADP release measured by single turnover (sequential mixing with dmantATP and then actin).