Table 1.
Monosaccharide composition of axy3 mutants
| Monosaccharides’ composition (μg mg−1)a | ||||||||
|---|---|---|---|---|---|---|---|---|
| Treatment | Rhamnose | Fucose | Arabinose | Xylose | Mannose | Galactose | Glucose | |
| I. TFA hydrolysis of AIRb | ||||||||
| Col0 | 25.8 ± 1.5 | 6.5 ± 0.3 | 36.0 ± 2.1 | 27.2 ± 1.6 | 12.7 ± 0.6 | 56.8 ± 3.2 | 26.8 ± 1.2 | |
| axy3.1 | 26.5 ± 1.5 | 5.5 ± 0.3 | 33.2 ± 0.6 | 27.5 ± 0.6 | 12.2 ± 0.7 | 54.0 ± 0.9 | 25.8 ± 0.6 | |
| axy3.2 | 25.9 ± 0.9 | 5.8 ± 1.4 | 34.2 ± 1.4 | 29.0 ± 1.2 | 12.7 ± 0.5 | 58.0 ± 2.4 | 28.6 ± 1.0 | |
| axy3.3 | 25.5 ± 1.4 | 6.0 ± 1.7 | 33.6 ± 1.7 | 28.4 ± 1.4 | 12.4 ± 0.6 | 62.4 ± 3.0 | 41.0 ± 2.1 | |
| pXYL1::XYL1 | 27.6 ± 1.8 | 6.2 ± 2.1 | 36.1 ± 2.1 | 26.5 ± 1.7 | 13.3 ± 1.0 | 57.8 ± 3.5 | 29.5 ± 1.9 | |
| II. Saeman hydrolysis of residue after TFA hydrolysisb | ||||||||
| Col0 | 0.4 ± 0.1 | ND | ND | 2.3 ± 0.2 | 7.6 ± 0.1 | 0.7 ± 0.1 | 163.9 ± 7.2 | |
| axy3.1 | 0.5 ± 0.0 | ND | ND | 2.1 ± 0.3 | 7.5 ± 0.4 | 0.7 ± 0.1 | 166.5 ± 6.0 | |
| axy3.2 | 0.4 ± 0.0 | ND | ND | 1.9 ± 0.2 | 7.3 ± 0.5 | 0.4 ± 0.0 | 176.6 ± 5.9 | |
| axy3.3 | 0.4 ± 0.0 | ND | ND | 1.8 ± 0.3 | 7.3 ± 0.6 | 0.5 ± 0.1 | 162.5 ± 10.0 | |
| pXYL1::XYL1 | 0.4 ± 0.0 | ND | ND | 2.5 ± 0.2 | 8.4 ± 0.3 | 0.4 ± 0.1 | 183.5 ± 8.0 | |
| III. Saeman hydrolysis of AIRb | ||||||||
| Col0 | 13.5 ± 1.9 | 4.1 ± 0.3 | 28.1 ± 3.0 | 19.8 ± 2.0 | 17.8 ± 1.4 | 64.0 ± 6.4 | 176.5 ± 18.1 | |
| axy3.1 | 12.0 ± 0.6 | 3.6 ± 0.3 | 26.7 ± 3.3 | 19.2 ± 2.8 | 16.3 ± 0.9 | 52.7 ± 1.3 | 183.6 ± 4.3 | |
| axy3.2 | 12.2 ± 0.1 | 3.6 ± 0.3 | 27.7 ± 0.4 | 19.4 ± 2.8 | 16.7 ± 0.2 | 56.1 ± 1.2 | 186.8 ± 2.5 | |
| axy3.3 | 11.2 ± 1.3 | 4.1 ± 0.4 | 28.8 ± 2.8 | 22.1 ± 3.0 | 17.3 ± 1.1 | 60.0 ± 4.2 | 180.0 ± 11.7 | |
| pXYL1::XYL1 | 13.7 ± 1.5 | 4.1 ± 0.5 | 30.6 ± 3.8 | 19.2 ± 2.7 | 18.5 ± 1.5 | 68.4 ± 6.9 | 178.7 ± 17.6 | |
| IV. Saeman hydrolysis of residue after buffer extractionb | ||||||||
| Col0 | 10.6 ± 0.8 | 3.7 ± 0.3 | 23.3 ± 2.8 | 17.5 ± 2.6 | 17.3 ± 1.3 | 48.0 ± 4.6 | 166.1 ± 12.5 | |
| axy3.1 | 8.7 ± 0.8 | 2.9 ± 0.1 | 20.4 ± 3.0 | 15.9 ± 2.8 | 14.7 ± 1.1 | 42.9 ± 4.8 | 177.0 ± 18.2 | |
| axy3.2 | 10.1 ± 1.6 | 3.1 ± 0.5 | 21.7 ± 3.5 | 16.1 ± 2.9 | 16.0 ± 2.0 | 45.8 ± 6.6 | 164.0 ± 23.2 | |
| axy3.3 | 8.6 ± 1.0 | 3.3 ± 0.4 | 20.5 ± 2.2 | 16.0 ± 2.2 | 15.3 ± 1.4 | 38.4 ± 3.6 | 159.6 ± 14.5 | |
| pXYL1::XYL1 | 9.6 ± 1.4 | 3.5 ± 0.5 | 22.8 ± 3.4 | 14.5 ± 2.4 | 15.4 ± 1.8 | 47.1 ± 6.2 | 153.2 ± 20.8 | |
| V. Saeman hydrolysis of residue after 4 M KOH extractionb | ||||||||
| Col0 | 5.9 ± 0.1 | 1.1 ± 0.2 | 13.7 ± 2.2 | 4.0 ± 0.9 | 5.4 ± 0.4 | 42.0 ± 2.0 | 137.2 ± 4.7 | |
| axy3.1 | 6.2 ± 0.8 | 1.0 ± 0.0 | 13.4 ± 2.1 | 3.7 ± 0.5 | 5.6 ± 0.4 | 32.4 ± 2.8 | 152.3 ± 9.7 * | |
| axy3.2 | 6.5 ± 0.5 | 1.0 ± 0.2 | 13.7 ± 1.3 | 3.4 ± 0.7 | 5.8 ± 0.6 | 36.9 ± 0.6 | 149.3 ± 7.0 * | |
| axy3.3 | 6.5 ± 0.6 | 1.2 ± 0.2 | 15.0 ± 1.8 | 4.3 ± 0.9 | 6.7 ± 0.7 | 40.6 ± 1.8 | 154.2 ± 2.1 * | |
| pXYL1::XYL1 | 6.2 ± 0.6 | 1.2 ± 0.2 | 14.3 ± 1.5 | 4.4 ± 1.0 | 6.0 ± 0.5 | 31.4 ± 0.6 | 141.4 ± 1.8 | |
| VI. Saeman hydrolysis of residue after XEG digestion of AIR followed by 4 M KOH extractionb | ||||||||
| Col0 | 7.0 ± 0.6 | 1.1 ± 0.2 | 13.3 ± 2.3 | 4.4 ± 1.2 | 6.5 ± 0.6 | 25.6 ± 1.9 | 164.2 ± 7.9 | |
| axy3.1 | 5.3 ± 0.6 | 1.1 ± 0.2 | 9.5 ± 1.3 | 3.2 ± 0.2 | 5.8 ± 0.6 | 18.1 ± 1.8 | 150.7 ± 7.9 | |
| axy3.2 | 5.9 ± 0.5 | 0.9 ± 0.1 | 10.0 ± 1.0 | 3.4 ± 0.5 | 6.4 ± 0.3 | 20.9 ± 0.9 | 159.8 ± 5.1 | |
| axy3.3 | 5.6 ± 0.5 | 0.9 ± 0.1 | 10.0 ± 0.8 | 3.2 ± 0.6 | 7.0 ± 0.5 | 22.5 ± 1.4 | 162.2 ± 5.7 | |
| pXYL1::XYL1 | 5.6 ± 0.4 | 0.9 ± 0.1 | 9.5 ± 0.8 | 3.3 ± 0.6 | 7.0 ± 0.5 | 20.3 ± 0.5 | 161.9 ± 8.2 | |
aThe mean value of 4–6 replicates (±SD) is shown. Asterisks indicate consistent significant differences (Student’s t test, p = 0.05) for axy3 mutants compared to Col0 and complemented axy3.1 line. ND not detected
bRoman numerals refer to the extraction scheme in Fig. S2