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. 2001 Mar 13;98(6):3304–3309. doi: 10.1073/pnas.051634098

Figure 1.

Figure 1

Mutated human IL-18 fusion proteins schema. The His-6 tag indicates the location of the six histidines fused in the N terminus of the IL-18 precursor propiece. The arrow indicates the ICE cleavage site as replaced by the factor Xa cleavage site (X). WT indicates wild-type mature IL-18. E42A indicates Glu-42 to Ala, K89A indicates Lys-89 to Ala, and E42A + K89A indicates the double mutation. On the basis of WT, three IL-18 mutants (E42A, K89A, and E42A + K89A) were generated by two-step PCR.