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Induction of IL-8 by IL-18 mutants. PBMCs were incubated with 30 ng/ml of either WT IL-18 or mutants. Polymyxin B (1 μg/ml) was mixed with IL-18 for 30 min before being added to the PBMCs. After 24 h, the supernatants were removed and assayed for IL-8 concentrations by ECL. One of three experiments is shown.
