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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Circ Cardiovasc Genet. 2010 Feb 2;3(2):187–198. doi: 10.1161/CIRCGENETICS.109.904813

Figure 6. Chromatin isolation from human TH promoter-transfected PC12 cells: Presence of the transfected plasmid in the chromatin fraction of the cell.

Figure 6

One day after transfection of the human TH-haplotype-1 promoter/luciferase reporter plasmid, subcellular fractionation was performed, and equal amounts (2.18 μg protein) of each subcellular fraction were used as template for PCR, over 15, 20 or 25 cycles, using an amplicon spanning TH promoter polymorphism C-824T. Results from 25 PCR cycles are shown. The PCR positive control plasmid was the human TH-haplotype-1 promoter/luciferase reporter (10 ng transfected). The chromatin isolation protocol was from the literature10.