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Colocalization of EGFP and cardiac proteins after transplantation of pre-incubated VSEL-SCs. VSEL-SC-derived cells are identified by EGFP immunoreactivity [(B)–(D), green]. Cells with possible cardiogenic commitment are identified by α-sarcomeric actin immunoreactivity [(C), (D), red]. (D) shows the merged image. Two cells are shown that are positive for both EGFP and α-sarcomeric actin (arrowheads, D, yellow).
