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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Int J Cancer. 2010 Nov 12;129(3):636–647. doi: 10.1002/ijc.25693

Figure 2.

Figure 2

CD8+Foxp3+TILs express surface markers and cytokines associated with effector function. a, Cells were analyzed by flow cytometry. Shaded histograms = Foxp3− subsets. Open histograms = Foxp3+ subsets. Foxp3+ cells showed higher expression of CD25, GITR and CTLA4 then Foxp3− cells. b, Foxp3 expression and IFN-γ secretion were determined by ICS following in vitro stimulation with plate-bound anti-CD3 or RNEU(420–429)-pulsed APCs. The percent IFN-γ+ was determined by subtracting the percent IFN-γ+ in the unstimulated sample (or irrelevant peptide) from the percent IFN-γ+ in the stimulated sample (or RNEU(420–429) peptide). The graphs are the compiled data of more than six independently conducted experiments. Error bars = standard error of the mean, NS = p > 0.05.