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Foxp3 expression in CD8+ T cells is dependent on the cytokine milieu. a, TILs were cultured with anti-CD3/CD28 beads with either IL-2 or TGF-β for 1 week. The percentages of each T cell subset was determined by flow cytometry. b, Naïve RNEU(420–429) peptide-specific T cells were stimulated with anti-CD3/CD28 beads and 5ng/ml of TGF-β alone or TGF-β + 20units/ml of IL-2.
