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. 2011 Feb;3(1):136–141. doi: 10.1111/j.1758-2229.2010.00199.x

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Journal compilation © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd

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Fig. 1 — Transformation of adenylate nucleotides and adenylate energy charge (AEC) in washed, pigmented cell suspensions (see Supporting information S2 for more detail) of Dinoroseobacter shibae (OD₄₃₆ = 10). To measure the AEC, a modified luciferin-luciferase assay was used which allows a precise quantification of ATP, ADP and AMP in a single assay (see Supporting information S5 for more detail). Cells were pre-incubated under anoxic conditions in the dark for 2 h. Upon addition of oxygen, fast ATP () formation occurred. The AMP (▴) concentration decreased correspondingly, while ADP (○) increased only transiently. This results in an increase of the energy charge (dashed line). All values are averages from two independent measurements.

Inline graphic — Transformation of adenylate nucleotides and adenylate energy charge (AEC) in washed, pigmented cell suspensions (see Supporting information S2 for more detail) of Dinoroseobacter shibae (OD₄₃₆ = 10). To measure the AEC, a modified luciferin-luciferase assay was used which allows a precise quantification of ATP, ADP and AMP in a single assay (see Supporting information S5 for more detail). Cells were pre-incubated under anoxic conditions in the dark for 2 h. Upon addition of oxygen, fast ATP () formation occurred. The AMP (▴) concentration decreased correspondingly, while ADP (○) increased only transiently. This results in an increase of the energy charge (dashed line). All values are averages from two independent measurements.