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. Author manuscript; available in PMC: 2012 Mar 11.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Feb 3;406(2):177–182. doi: 10.1016/j.bbrc.2011.01.113

Fig. 2. Inhibition of ornithine decarboxylaseby yeast antizyme and affinity constants of protein-protein interaction.

Fig. 2

(A) 2 pmole of ODC was incubated in the presence and absence of antizyme, and the ODC activity remaining after incubation was determined by 14CO2 trapped in Ba(OH)2 soaked filter paper. Assays were performed in triplicates and the average data are presented here. (B) Sensogram showing the binding of different concentration of ODC protein (0.125, 0.25 and 0.5 µM) to the surface immobilized antizyme protein, as obtained from surface plasmon resonance analyses in a Biacore 2000 as described in Materials and Methods.