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. Author manuscript; available in PMC: 2012 Mar 11.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Feb 3;406(2):177–182. doi: 10.1016/j.bbrc.2011.01.113

Fig. 3. Stoichiometry of yeast ODC:AZ complex.

Fig. 3

(A) Purified ODC was incubated overnight with His-tagged purified antizyme in different molar ratios as indicated in the figure. The resulting complex was trapped on cobalt (TALON) column and purified as described in Materials and Methods. An aliquot of the purified sample was run on SDS-PAGE together with protein standard. (B). The purified ODC: AZ complex was assayed on SEC-lightscattering to determine the native molecular weight of the ODC: AZ complex. The top panel is showing the dynamic (multi angle) light scattering, the bottom panel is showing the refractive index for protein concentration determination as determined by a refractive index detector (Optilab Rex).