Skip to main content
. Author manuscript; available in PMC: 2012 Mar 11.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Feb 3;406(2):177–182. doi: 10.1016/j.bbrc.2011.01.113

Fig. 4. CD spectra of purified AZ, ODC and ODC: AZ complex show a change in secondary structure in the complex.

Fig. 4

Far UV (260–190 nm) CD spectra of purified antizyme (A) and ornithine decarboxylase (B) proteins. Antizyme or ornithine decarboxylase proteins at 0.1 mg/ml were run on a CD (JASCO) and the mean ellipticity of the molecule was determined. The data was analyzed to calculate the percentage of α-helices and β-sheets present in antizyme protein as mentioned in the text. (C). Far UV (260–190 nm) CD spectra of purified ODC: AZ complex (0.1 mg/ml) is shown here. The data were calculated and compared with the sum of the CD spectra of 1:1 molar ratios two molecules.