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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Dev Biol. 2011 Jan 14;352(1):154–163. doi: 10.1016/j.ydbio.2011.01.007

Figure 6. Targeted disruption of GATAa activity perturbs posterior endoderm morphogenesis.

Figure 6

(A–F) Partial lateral projections of representative embryos co-electroporated with Mesp-lacZ to mark the heart progenitors (blue), Ttf-GFP-strabismus to mark the endoderm (green), and either Ttf-GATAa-DBD (A–C’) or Ttf-GATAa-WRPW (D–F’). Embryos were fixed at the stage indicated at the top of the figure. Note that GFP-strabismus was enriched basolateraly in polarized epithelia as observed previously (Gravel et al., 2010). (A’–F’) To clarify endoderm morphology, schematic representations of corresponding embryos (A–F) were created using single optical sections including F-actin staining (grey) with the GFP-strabismus expressing endoderm cells pseudo-colored (yellow = ventral floor, red posterior endoderm). White arrow indicates the endodermal strand. Scale bar: 10 µm.